Detection of CC17 Enterococcus faecium in dogs and a comparison with human isolates     

Kwon KH  Moon BY  Hwang SY  Park YH 《Zoonoses and public health》2012,59(6):375-378

Enterococcus faecium strains of clonal complex (CC) 17 were isolated from domestic dogs. The strains were more prevalent in infectious isolates than in colonized isolates, suggesting that strains of the CC17 lineage may have an advantage in causing infections in dogs. The pulsed field gel electrophoresis patterns of some dog and human isolates were over 90% similar. However, antimicrobial resistance patterns and virulence factors were not identical, which might reflect different use of antimicrobials in veterinary medicine or in host specificity.  相似文献   

致鹅败血症粪肠球菌的分离鉴定     

狄婷婷  高原  聂鑫  马万欣  董国军  曾钰然 《中国预防兽医学报》2012,34(3):192-196

为确定导致通辽某鹅场雏鹅败血症的病原,本研究将从病鹅心、肝、淋巴结和脑组织液中分离的革兰氏阳性球菌纯化培养,进行形态学和生长耐受性观察,菌属区别、分群和菌种鉴定试验,16S rDNA检测、系统进化分析和致病性试验。结果表明,分离菌株(HQ831431)为中等致病力粪肠球菌,具有β型溶血特性,可致小鼠急性败血症。分离菌株与参考菌株ATCC29212及国内外分离的其他15株粪肠球菌16S rDNA的同源性均在99.0%以上,位于系统发育树的同一分支。  相似文献   

蜡蚧轮枝菌拮抗菌——粪产碱杆菌生物学特性的初步研究     

李美  张龙 《福建农林大学学报(自然科学版)》2009,38(1)

分离到了一种对蜡蚧轮枝菌具有明显抑制作用的细菌,采用生理生化反应方法对该细菌进行种类鉴定.结果表明,该细菌为粪产碱杆菌(Alcaligenes faecalis)菌株.在不同温度下对该细菌的生长速度和对不同抗生素的敏感性进行研究,发现在弱酸性条件下,该菌生长最快的温度为30-39℃,对卡那霉素、氯霉素、四环素、链霉素都敏感,对卡那霉素的完全抑制最低浓度为60μg.mL-1.  相似文献   

乳酸菌制剂在黑毛和牛育肥中的使用效果     

袁清珠  北村良久  深田一刚  岛田贵志  能味堂朗 《中国牛业科学》2010,36(1):23-29

[目的]旨在通过黑毛和牛（Japanese Blackcattle）育肥后期投服乳酸菌制剂Entero—coccus faecalis FK-23粉末,探讨付与牛肉的附加价值和该制剂的实用性。[方法]供试牛10头,随机分为试验组和对照组,试验组5头,每头每日投服乳酸菌制剂8．0g。测量体重（BW）,静脉采血检测白细胞数（WBC）,维生素A（VA）,维生素E（VE）．总胆固醇（TC）,谷草转氨酶（GOT）,血尿素氮（BUN）及血糖（GLC）浓度;HPLC测验中性脂肪的脂肪酸成分。[结果]试验组与对照组比较。食欲亢进,日增重（DG）明显增加（P〈0．05）,血清VE和TC明显升高（P〈0．05）,VA维持预计水平走向不变。中性脂肪的不饱和脂肪酸比例出现增高趋势,出栏体重增长4．3％,胴体重增加9．3kg。[结论]结果提示,黑毛和牛育肥后期投服乳酸菌E．faecalisFK-23制剂可以获得VE含量较高的高附加价值牛肉并能提高其生产性能。  相似文献   

Distribution of streptogramin resistance genes and genetic relatedness among quinupristin/dalfopristin-resistant Enterococcus faecium recovered from pigs and chickens in Korea     

I.Y. Hwang  S.K. Lim  C.K. Park  S.C. Jung  H.M. Nam 《Research in veterinary science》2010,89(1):1-1355

Fifty-four quinupristin/dalfopristin-resistant Enterococcus faecium (QDREF) isolated from chickens and pigs during 2002-2003 in Korea were screened by PCR for the presence of streptogramin resistance genes vatD, vatE, and vgbA, and macrolide resistance gene ermB. None of the QDREF isolates carried vgbA and vatD genes, while vatE and ermB were detected in 9.2% and 74% of the isolates, respectively. Twenty-six percent (14/54) of the QDREF isolates contained none of the resistance determinants tested. Pulsed-field gel electrophoresis (PFGE) patterns revealed high heterogeneity: 47 different patterns for 54 QDREF evaluated. Identical PFGE types were observed in two pairs of chicken isolates and a pair of pig isolates, respectively, but chicken isolates did not share PFGE pattern with pig isolates, suggesting clonal spread of QDREF strain between the same species of animals but not between different species of animals. This is the first report, to our knowledge, of vatE-positive E. faecium isolates and also the first evidence of clonal spread of QDREF strain between animals in Korea.  相似文献   

3种不同水源粪肠球菌耐药情况     

易秀丽  俞道进  黄一帆 《福建农林大学学报(自然科学版)》2008,37(1):92-95

为了解水环境中粪肠球菌对常用抗生素的耐药情况,从某校鱼塘、污水沟和模型池塘中共分离出99株粪肠球菌,采用美国临床实验室标准委员会(NCCLS)推荐的微量肉汤稀释法进行药敏试验.结果显示:污水沟分离菌的耐药率最高,其中红霉素、土霉素、环丙沙星、氯霉素和氨苄西林的耐药率分别为89.3%、89.3%、82.1%、46.4%和32.1%,且多数对3-5种抗生素多重耐药;其次为鱼塘分离菌,耐药谱多为对1-2种抗生素同时耐药;模型池塘分离敏感菌较多;3处均未分离出万古霉素耐药粪肠球菌.提示不同水源粪肠球菌耐药情况与水源受抗生素污染程度存在联系,表明细菌耐药性监测及环境保护的重要性.  相似文献   

不同肠球菌基因组DNA提取方法对PCR鉴定的影响     

张祥  李晓博  谷素美  段志刚  王亚宾  胡慧  陈丽颖 《河南农业大学学报》2012,46(1):67-71

为研究肠球菌基因组DNA的不同提取方法对16SrRNA基因序列PCR、种属多重PCR、RAPDPCR等肠球菌鉴定结果的影响,分别采用6种基因组DNA提取方法(水煮法1、水煮法2、常规酚/氯仿抽提法、chelex-100法1、chelex-100法2和试剂盒法),对1株肠球菌标准菌株ATCC29212基因组DNA进行提取,以这些分别提取的DNA为模板在相同的扩增条件下进行PCR检测.结果表明,在16SrRNA基因序列PCR和种属多重PCR检测中以水煮法1进行肠球菌DNA的提取最适宜,而试剂盒法在RAPDPCR检测中效果最佳.  相似文献   

一例肉鸡大肠杆菌和粪肠球菌混合感染的诊治     

管夕柱  李开锋  张启迪  刘文华 《中国畜牧兽医》2012,39(8):223-226

本试验对某发病肉鸡场的病死鸡进行了病原学诊断。通过病原分离、生化试验、动物试验、血清型鉴定、PCR扩增等方法对分离细菌进行了鉴定,最终确定为大肠杆菌和粪肠球菌的混合感染。在此基础上进行了药敏试验,筛选出了大肠杆菌的高敏药物阿米卡星和庆大霉素,而粪肠球菌只对某些氟喹诺酮类药物中度敏感,其余均显示为耐药。将阿米卡星、庆大霉素和恩诺沙星用于发病鸡群的治疗,很快控制了疫情,及时减少了经济损失。  相似文献   

一株猪源屎肠球菌的分离、鉴定及益生功能初步研究   总被引：1，自引：1，他引：0  

王凡  王建设  刘婷  李晓清  王安如 《中国畜牧兽医》2012,39(7):231-234

从健康仔猪粪便中分离到一株乳酸菌ZW002,并从形态、生理生化指标,16S rRNA基因序列分析,鉴定该菌株为屎肠球菌(Enterococcus faecium),将其16S rRNA基因提交GenBank,登录号为JN542513。从耐酸、耐胆盐及抑菌性能等方面对菌株进行益生功能初步研究,结果表明,菌株ZW002表现出良好的耐受性及益菌性能,为猪用微生态制剂的研究开发奠定了基础。  相似文献   

Isolation，Identification and Antibiotics Resistance Analysis of Enterococcus from Yak     

XU Feng  ZHANG Huan-rong  LIANG Hua  ZHU Ming-xin  TANG Cheng 《中国畜牧兽医》2017,44(4):1203-1210

17 feces samples of yak which were collected in Hongyuan county were measured with Gram staining method and 16S rRNA molecular identification in this study.8 suspected Enterococcus were separate from feces samples by bacteria purification and PCR amplification with 1 500 bp specific band. 6Enterococcus faecalis and 2Enterococcus faecium were identified through 16S rRNA sequencing.The homology analysis of the strains revealed that the homology between Enterococcus faecalis and reference strains sequence were 99.7% to 100%,that of Enterococcus faecium and reference sequence were 98.2% to 99.2%,indicating that the yak Enterococcus was highly conserved in the process of genetic evolution.The drug sensitive test results showed that the isolated strains were highly resistance to aminoglycoside antibiotics.Enterococcus faecium 11-1-2 strain was not only 5 multi-resistant,but also showed resistence to vancomycin.Enterococcus faecalis strains was most 3 multi-resistant.The antibiotics resistance results revealed that the resistance of yak Enterococcus was serious and should be taken seriously.  相似文献